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Transfusion Medicine Reviews
Volume 21, Issue 3
, Pages 237-254
, July 2007
Real-Time Polymerase Chain Reaction in Transfusion Medicine: Applications for Detection of Bacterial Contamination in Blood Products
References
- . Bacterial contamination of blood components. Clin Microbiol Rev. 2005;18:195–204
- . Transfusion-associated bacterial sepsis. Clin Microbiol Rev. 1994;7:290–302
- . Determination of the degree of bacterial contamination of whole-blood collections using an automated microbe-detection system. Transfusion. 2001;41:815–818
- Transfusion transmitted bacterial infection in the United States, 1998 through 2000. Transfusion. 2001;41:1493–1499
- . Transfusion-transmitted bacterial infection: Risk, sources and interventions. Vox Sang. 2004;86:157–163
- Sterility testing of platelet concentrates prepared from deliberately infected blood donations. Transfusion. 2006;46:486–491
- Clinical significance of bacteriologic screening in platelet concentrates. Transfusion. 2005;45:514–519
- Bacterial contamination of platelet concentrates prepared by different methods: Results of standardized sterility testing in Germany. Vox Sang. 2006;90:177–182
- Growth of bacteria in inoculated platelets: Implications for bacteria detection and the extension of platelet storage. Transfusion. 2000;40:1308–1312
- . Two novel real-time reverse transcriptase PCR assays for rapid detection of bacterial contamination in platelet concentrates. J Clin Microbiol. 2004;42:4759–4764
- In-house validation of the BACTEC 9240 blood culture system for detection of bacterial contamination in platelet concentrates. Transfusion. 2005;45:1138–1142
- Enhancement of a culture-based bacterial detection system (eBDS) for platelet products based on measurement of oxygen consumption. Transfusion. 2005;45:984–993
- Evaluation of the Scansystem method for detection of bacterially contaminated platelets. Transfusion. 2005;45:265–269
- Detection of bacteria in platelet concentrates: Comparison of broad-range real-time 16S rDNA polymerase chain reaction and automated culturing. Transfusion. 2005;45:731–736
- . Perspectives and limitations in the bacterial screening of platelet concentrates. J Lab Med. 2006;30:60–65
- Basics of flow cytometry–based sterility testing of platelet concentrates. Transfusion. 2006;46:41–49
- . Methods for the detection of bacterial contamination in blood products. J Lab Med. 2006;30:74–90
- . High-volume extraction of nucleic acids by magnetic bead technology allows ultrasensitive detection of bacteria in blood components. Clin Chem. 2007;[in press]
- Elimination and multiplication of bacteria during preparation and storage of buffy coat–derived platelet concentrates. Transfusion. 2006;46:949–955
- Real-time PCR in clinical microbiology: Applications for routine laboratory testing. Clin Microbiol Rev. 2006;19:165–256
- . Quantification using real-time PCR technology: Applications and limitations. Trends Mol Med. 2002;8:257–260
- The real-time polymerase chain reaction. Mol Aspects Med. 2006;27:95–125
- . Nucleic acid–based methods for the detection of bacterial pathogens: Present and future considerations for the clinical laboratory. Clin Chim Acta. 2006;363:206–220
- . Real-time PCR in the microbiology laboratory. Clin Microbiol Infect. 2004;10:190–212
- . Real-time PCR. Encyclopedia of diagnostic genomics and proteomics. 2005;11:17–25
- Chimeric LNA/DNA probes as a detection system for real-time PCR. Clin Biochem. 2004;37:930–932
- Evaluation of the performance of LNA and MGB probes in 5'-nuclease PCR assays. Mol Cell Probes. 2003;17:307–311
- . Detection and monitoring of virus infections by real-time PCR. Mol Aspects Med. 2006;27:245–298
- . Rapid identification of Yersinia enterocolitica in blood by the 5′ nuclease PCR assay. J Clin Microbiol. 2000;38:1953–1958
- . Multiplex PCR for detection of Enterobacteriaceae in blood. Transfusion. 2001;41:1356–1364
- . Direct identification of Yersinia enterocolitica in blood by polymerase chain reaction amplification. Transfusion. 1992;32S:850–854
- Detection of nucleic acid sequences from bacterial species with molecular genetic methods. Transfus Sci. 2000;23:21–27
- . Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service. J Med Microbiol. 2003;52:685–691
- . Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J Mol Diagn. 2005;7:575–581
- Detection of bacterial DNA in blood samples from febrile patients: Underestimated infection or emerging contamination?. FEMS Immunol Med Microbiol. 2004;42:249–253
- Use of tuf sequences for genus-specific PCR detection and phylogenetic analysis of 28 streptococcal species. J Clin Microbiol. 2004;42:3686–3695
- rpoB gene sequence-based identification of aerobic Gram-positive cocci of the genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella. J Clin Microbiol. 2004;42:497–504
- HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol. 1996;34:818–823
- Bacterial phylogeny based on comparative sequence analysis. Electrophoresis. 1998;19:554–568
- 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991;173:697–703
- . Rapid identification of bacteria on the basis of polymerase chain reaction–amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol. 1993;59:945–952
- . Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species. Appl Environ Microbiol. 1995;6-1:2798–2801
- . New approaches to typing and identification of bacteria using 16S-23S rDNA spacer region. Microbiology. 1996;142:3–116
- Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology. 2002;148:257–266
- In: Molecular biology of the gene. vol 1:Menlo Park (CA): The Benjamin/Cummings Publishing Company; 1987;p. 466
- . Molecular methods for the assessment of bacterial viability. J Microbiol Methods. 2003;53:175–183
- . Functional mRNA half lives in E. coli. Mol Gen Genet. 1978;166:329–336
- Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A. 2002;99:9697–9702
- Detection of mRNA by reverse transcription–PCR as an indicator of viability in Escherichia coli cells. Appl Environ Microbiol. 1998;64:1313–1318
- Detection of bacteremia in emergency department patients at risk for infective endocarditis using universal 16S rRNA primers in a decontaminated polymerase chain reaction assay. J Infect Dis. 2002;186:1677–1681
- New developments in the diagnosis of bloodstream infections. Lancet Infect Dis. 2004;4:751–760
- PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditis. J Clin Microbiol. 2005;43:163–167
- Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing. J Clin Microbiol. 1997;35:2733–2739
- Impact of a molecular approach to improve the microbiological diagnosis of infective heart valve endocarditis. Circulation. 2005;111:1415–1421
- Prospective study of use of PCR amplification and sequencing of 16S ribosomal DNA from cerebrospinal fluid for diagnosis of bacterial meningitis in a clinical setting. J Clin Microbiol. 2004;42:734–740
- Employment of broad range 16S rDNA PCR and sequencing in the detection of aetiological agents of meningitis. New Microbiol. 2005;10 28:135–143
- . Diagnostic PCR: validation and sample preparation are two sides of the same coin. APMIS. 2004;112:808–814
- . In-house nucleic acid amplification assays in research: How much quality control is needed before one can rely upon the results?. J Clin Microbiol. 2005;43:5835–5841
- . ICH topic Q2A: Validation of analytical methods: Definitions and terminology. CPMP/ICH/381/95. http://www.eudra.org/eudraportal/
- . ICH topic Q2B: Validation of analytical methods: Methodology. CPMP/ICH/281/95. http://www.eudra.org/eudraportal/
- Anonymous. European Pharmacopeia: PCR monograph
- . Standardization: A progress report. Biologicals. 1999;27:285–289
- . Validation and standardisation of nucleic acid amplification technology (NAT) assays for the detection of viral contamination of blood and blood products. J Clin Virol. 2001;20:7–13
- . Concepts for bacterial safety of blood components in Germany. Transfus Apher Sci. 2001;24:251–252
- Optimized Scansystem platelet kit for bacterial detection within 24 h after spiking. Vox Sang. 2005;89:135–139
- Detection of bacteria in platelet concentrates prepared from spiked single donations using cultural and molecular genetic methods. Transfus Med. 2007;17:61–70
- . Mycoplasmas (Mollicutes) have a low number of rRNA genes. J Bacteriol. 1984;158:376–378
- Removal of contaminating DNA from commercial nucleic acid extraction kit reagents. J Microbiol Methods. 2005;61:285–288
- . Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR. Appl Environ Microbiol. 2001;67:3985–3993
- . Use of bacteriophage MS2 as internal control in viral RT-PCR assays. J Clin Microbiol. 2005;43:4551–4557
- A novel RT-PCR for reliable and rapid HCV RNA screening of blood donations. Transfusion. 2001;41:1100–1106
- . Electrochemiluminescent detection of bacteria in blood components. Transfus Med. 2002;12:115–123
- . Direct detection of bacteria in cellular blood products using bacterial ribosomal RNA–directed probes coupled to electrochemiluminescence. Transfus Med. 1999;9:177–188
- . Platelet bacterial contamination and the use of a chemiluminescence-linked universal bacterial ribosomal RNA gene probe. Transfusion. 1994;34:750–755
- . The use of a chemiluminescence-linked universal bacterial ribosomal RNA gene probe and blood gas analysis for the rapid detection of bacterial contamination in white cell reduced and nonreduced platelets. Transfusion. 1993;33:450–457
- Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates. J Clin Microbiol. 2003;41:4796–4798
- Detection and differentiation of in vitro–spiked bacteria by real-time PCR and melting-curve analysis. J Clin Microbiol. 2004;42:512–517
- Presence of Propionibacterium acnes in blood components. Transfusion. 2001;41:1126–1129
- PCR evaluation of false-positive signals from two automated blood-culture systems. J Med Microbiol. 2006;55:53–57
- Rapid identification of bacteria from positive blood cultures by fluorescence-based PCR–single-strand conformation polymorphism analysis of the 16S rRNA gene. J Clin Microbiol. 2000;38:513–520
- Optimal sampling time after preparation of platelet concentrates for detection of bacterial contamination by quantitative real-time polymerase chain reaction. Vox Sang. 2005;89:208–214
- . Improving the bacteriological safety of platelet transfusion. Transfus Med Rev. 2004;18:11–24
- . Bacterial contamination in blood components and preventative strategies: An overview. Transfus Apher Sci. 2004;31:155–163
- Staphylococcus epidermidis bacteremia from transfusion of contaminated platelets: application of bacterial DNA analysis. Transfusion. 1992;32:771–774
- Bacterial proliferation in platelet concentrates. Transfusion. 1986;26:388–390
- Rapid screening method for detection of bacteria in platelet concentrates. J Clin Microbiol. 2004;42:1903–1908
- Operational feasibility of routine bacterial monitoring of platelets. Transfus Med. 2003;13:189–195
- Detection of bacteria in WBC-reduced PLT concentrates using percent oxygen as a marker for bacteria growth. Transfusion. 2003;43:1276–1283
- Evaluation of the enhanced bacterial detection system for screening of contaminated platelets. Transfusion. 2006;46:220–224
- . Optimal DNA isolation method for detection of bacteria in clinical specimens by broad-range PCR. J Clin Microbiol. 2002;40:4211–4217
- . Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR. J Clin Microbiol. 2005;43:5541–5546
- Evaluation of an automated high-volume extraction method for viral nucleic acids in comparison to a manual procedure with preceding enrichment. Vox Sang. 2005;89:71–76
- Magnetic bead technology in viral RNA and DNA extraction from plasma minipools. Transfusion. 2005;45:1106–1110
- . Efficient extraction of viral DNA and viral RNA by the Chemagic viral DNA/RNA Kit allows sensitive detection of cytomegalovirus, hepatitis B virus, and hepatitis G virus by PCR. J Clin Microbiol. 2003;41:5273–5276
- . Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology. J Clin Microbiol. 2002;40:1575–1580
- . Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 1990;93:125–128
- . Frequent contamination of Taq polymerase with DNA. Clin Chem. 1990;36:1258
- . Taq polymerase contains bacterial DNA of unknown origin. Mol Cell Probes. 1990;4:445–450
- Contamination and sensitivity issues with a real-time universal 16S rRNA PCR. J Clin Microbiol. 2000;38:1747–1752
- . Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion. J Clin Microbiol. 1999;37:3402–3404
- . Elimination of contaminating DNA within polymerase chain reactions reagents: Implications for a general approach to detection of uncultured pathogens. J Clin Microbiol. 1993;31:646–652
- . Use of psoralen as extinguisher of contaminated DNA in PCR. Nucleic Acids Res. 1990;18:6739
- . Strategies for the avoidance of bacterial contamination of blood components. Transfusion. 1993;33:228–233
- Platelet transfusion–related sepsis. Aust N Z J Med. 1993;23:717
PII: S0887-7963(07)00021-1
doi: 10.1016/j.tmrv.2007.03.006
© 2007 Elsevier Inc. All rights reserved.
« Previous
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Transfusion Medicine Reviews
Volume 21, Issue 3
, Pages 237-254
, July 2007
