Transfusion Medicine Reviews
Volume 11, Issue 3 , Pages 224-233 , July 1997

Parameters of cell freezing: Implications for the cryopreservation of stem cells

  • A. Hubel

      Affiliations

    • Corresponding Author InformationAddress reprint requests to Allison Hubel, PhD, Biomedical Engineering Center, University of Minnesota, Box 107 UMHC, Minneapolis, MN 55455.

References 

  1. Barnes DWH, Loutit JF. The radiation recovery factor: Preservation by the Polge-Smith-Parkes technique. J Natl Cancer Inst. 1955;15:901
  2. Areman E, Sacher R, Deeg H. Processing and storage of human bone marrow: A survey of current practices in North America. Bone Marrow Transplant. 1990;6:203–209
  3. Rowley SD. Hematopoietic stem cell cryopreservation: A review of current techniques. J Hematother. 1992;1:233–250
  4. Lasky L, VanBuren N, Weisdorf D, et al. Successful allogeneic cryopreserved marrow transplantation. Transfusion. 1989;29:182–189
  5. Amos T, Gordon M. Sources of human hematopoietic stem cells for transplantation. Cell Transplant. 1995;4:547–569
  6. Noga S, Davis J, Schepers K, et al. The clinical use of elutriation and positive stem cell selection columns to engineer the lymphocyte and stem cell composition of the allograft. Prog Clin Biol Res. 1994;389:317–324
  7. Ratajczak M, Ratajczak J, Kregenow D, et al. Growth factor stimulation of cryopreserved CD 34+ bone marrow cells intended for transplant: An in vitro study to determine optimal timing of exposure to early acting cytokines. Stem Cells. 1994;12:599–603
  8. Williams S, Lee W, Bender J, et al. Selection and expansion of peripheral blood CD34+ cells in autologous stem cell transplantation of breast cancer. Blood. 1996;87:1687–1691
  9. Young J, Bruno E, Luens K, et al. Thrombopoietin stimulaties megakaryocytopoiesis, myelopoiesis and expansion of CD34+ progenitor cells from single CD34+Thy−1 Lin−1 primitive progenitor cells. Blood. 1996;88:1619–1631
  10. Siena S, Di Nicola M, Bregni M, et al. Massive ex vivo generation of functional dendritic cells from mobilized CD34+ cells for anticancer therapy. Exp Hematol. 1995;23:1463–1471
  11. Dunbar C, Kohn D, Karlsson S, et al. Retroviral mediated transfer of the cDNA for human glucocerebrosidase into hematopoietic stem cells of patients with Gaucher disease. A phase I study. Hum Gene Ther. 1996;7:231–253
  12. Blaese R, Culver C, Chang K, et al. Treatment of severe combined immundeficiency (SCID) due to adenosine deaminase (ADA) deficiency with CD+ selected autologous peripheral blood stem cells transduced with the human ADA gene. Hum Gene Ther. 1993;4:521–527
  13. Douer D, Levine A, Anderson WF, et al. High-dose chemotherapy and autologous bone marrow plus peripheral blood stem cell transplantation for patients with lymphoma or metastatic breast cancer: Use of marker genes to investigate hematopoietic reconstitution in adults. Hum Gene Ther. 1996;7:669–684
  14. Sputtek A, Korber C. Cryopreservation of red blood cells platlets, lymphocytes and stem cells. In: Fuller BJ, Grout B editor. Clinical Applications of Cryobiology. Boca Raton, FL: CRC Press; 1991;p. 127–147
  15. Toner M. Nucleation of ice crystals inside biological cells. In: Steponkus P editors. Low-Temperature Biology. London, England: JAI Press; 1993;p. 1–51
  16. Lovelock JE. The haemolysis of human red blood cells by freezing and thawing. Biochem Biophys Acta. 1953;10:414–426
  17. Hubel A, Cravalho EG, Nunner B, et al. Survival of directionally solidified B-lymphoblasts under various crystal growth conditions. Cryobiology. 1992;29:183–198
  18. (suppl) Mazur P. Freezing of living cells: Mechanisms and implications. Am J Physiol. 1984;247:C125–C142
  19. Mazur P. Stopping biological time: The freezing of living cells. Ann NY Acad Sci. 1988;541:514–531
  20. Mazur P. The role of intracellular freezing in the death of cells cooled at supraoptimal rates. Cryobiology. 1977;14:251–272
  21. Mazur P, Schmidt J. Interactions of cooling velocity, temperature, and warming velocity on the survival of frozen and thawed yeast. Cryobiology. 1968;5:1–17
  22. Leibo S, Mazur P, Jackowski S. Factors affecting survival of mouse embryos during freezing and thawing. Exp Cell Res. 1974;89:79–88
  23. Leibo S, Farrant J, Mazur P, et al. Effect of freezing on marrow stem cell suspensions: Interactions of cooling and warming rates in the presence of PVP, sucrose or glycerol. Cryobiology. 1970;6:315–332
  24. Nei T. Mechanism of hemolysis of erythrocytes by freezing at near-zero temperatures I microscopic observation of hemolyzing erythrocytes during the freezing and thawing process. Cryobiology. 1967;4:153–156
  25. Pegg DE, Diaper MP, Skaer H, et al. The effect of cooling rate and warming rate on the packing effect of human erythrocytes frozen and thawed in the presence of 2 M glycerol. Crybiology. 1984;21:491–502
  26. Pegg DE, Diaper MP. The “unfrozen fraction” hypothesis of freezing injury to human erythrocytes: A critical examination of the evidence. Cryobiology. 1989;26:30–43
  27. Rowley SD, Bensinger W, Gooley T, et al. Effect of cell concentration on bone marrow and peripheral blood stem cell cryopreservation. Blood. 1994;83:2731–2736
  28. McGrath JJ. Membrane transport properties. In: McGrath JJ, Diller KR editor. Low Temperature Biotechnology. New York, NY: ASME; 1988;p. 273–330
  29. Levin RL, Cravalho EG, Huggins CE. A membrane model describing the effect of temperature on the water conductivity of erythrocyte membranes at subzero temperatures. Cryobiology. 1976;13:415–429
  30. Walcerz DB. Cryosim: A user-friendly program for simulating cryopreservation protocols. Cryobiology. 1995;32:35–51
  31. In: Areman E, Deeg H, Sacher R editor. Bone Marrow and Stem Cell Processing: A Manual of Current Techniques. ed 1. Philadelphia, PA: FA Davis; 1992;
  32. Rubenstein P, Dobrila L, Rosenfield R, et al. Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution. Proc Natl Acad Sci. 1995;92:10119–10122
  33. Karlsson JOM, Toner M. Long-term storage of tissues by cryopreservation: Critical issues. Biomaterials. 1996;17:243–256
  34. Hubel A, Norman JA, Darr TB: Cryobiophysical characteristics of genetically modified hematopoietic stem cells for the treatment of Mucopolysaccharidosis Type I. Cryobiology (submitted)
  35. Fahy GM. Analysis of solution effects injury. Biophys J. 1980;32:837–850
  36. Boutron P. Comparison with the theory of the kinetics and extent of ice crystallization and of the glass-forming tendency in aqueous cryoprotective solutions. Cryobiology. 1986;23:88–102
  37. Sakai A, Yoshida S. The role of sugar and related compounds in variation of freezing resistance. Cryobiology. 1968;5:160–174
  38. Levin RL, Miller TW. An optimum method for the introduction or removal of permeable cryoprotectants: Isolated cells. Cryobiology. 1981;18:32–48
  39. Goldman J, Th'ng K, Park D, et al. Collection, cryopreservation and subsequent viability of haemopoietic stem cells intended for treatment of chronic granulocytic leukaemia in blast-cell transformation. Br J Hematol. 1978;40:185–195
  40. Fahy G, Lilley T, Linsell H, et al. Cryoprotectant toxicity and cryoprotectant toxicity reduction: In search of molecular mechanisms. Cryobiology. 1991;27:247–268
  41. Douay L, Gorin N, David R, et al. Study of granulocyte-macrophage progenitor (CFU-c) preservation after slow freezing of bone marrow in the gas phase of liquid nitrogen. Exp Hematol. 1982;10:360–366
  42. Rowley SD, Anderson G. Effect of DMSO exposure without cryopreservation on hematopoietic progenitor cells. Bone Marrow Transplant. 1993;11:389–393
  43. Davis JM, Rowley SD, Braine HG, et al. Clinical toxicity of cryopreserved bone marrow graft infusion. Blood. 1990;75:781–786
  44. Stroncek D, Fautsch S, Lasky L, et al. Adverse reactions in patients transfused with cryopreserved marrow. Transfusion. 1991;31:521–526
  45. Stiff P, Murgo K, Zaroulis C, et al. Unfractionated human marrow cell cryopreservation using dimethylsulfoxide and hydroxyethyl starch. Cryobiology. 1983;20:17–24
  46. Kessler D, Siegel J, Noguchi P, et al. Regulation of somatic-cell and gene therapy by the Food and Drug Administration. N Engl J Med. 1993;329:1169–1173
  47. Antwiler G, Dumont L, Vandenbroeke T. The preparation and freezing of platelets using a transfusable cryoprotectant. In: Alving B editors. Frozen Platelets and Platelet Substitutes in Transfusion Medicine. Bethesda, MD: USAMRMC, NIH, and FDA; March 1996;p. 21
  48. Ashwood-Smith M, Morris G, Fowler R, et al. Physical factors are involved in the destruction of embryos and oocytes during freezing and thawing procedures. Hum Reprod. 1988;3:795–802
  49. Schwartz G, Diller K. Analysis of the water permeability of human granulocytes at subzero temperature in the presence of extracellular ice. J Biomech Eng. 1983;105:360–366
  50. Scheiwe MW, Korber C. Basic investigations on the freezing of human lymphocytes. Cryobiology. 1983;20:257–273
  51. McCaa C. Cryomicroscopic determination of the transient permability parameters of monocyte cells at sub-zero temperatures. In: Master's thesis. Austin, TX: University of Texas; December 1986;
  52. McGann L, Janowska-Wieczorek A, Turner A, et al. Water permeability of human hematopoietic stem cells. Cryobiology. 1987;24:112–119
  53. Branch DR, Calderwood S, Cecilitti MA, et al. Hemostatic progenitor cells are resistant to dimethyl sulfoxide toxicity. Transfusion. 1994;34:887–890

 Supported in part by National Institutes of Health Grant P01 HD 32652 and Baxter Healthcare Corporation.

PII: S0887-7963(97)80043-0

doi: 10.1053/tmrv.1997.0110224

Transfusion Medicine Reviews
Volume 11, Issue 3 , Pages 224-233 , July 1997